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The cDNA synthesis was made by reverse transcription using the High Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, USA) following manufacturers instructions and analyzed the expression of wound healing related genes (i.e., Col1a1 , Col3a1 , Fgf2 , Tgfb1 , Egf , and Il6 ) using SYBR Green-based qPCR

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